C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.1, 150/3.
C12Q 1/26 (2006.01) C12N 9/96 (2006.01) C12Q 1/28 (2006.01) C12Q 1/46 (2006.01) C12Q 1/60 (2006.01)
Patent
CA 1154366
Abstract of the Disclosure Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by dissolving a salt of cholic acid in a buffer solution providing a pH within the range of a?out 4 to about 9, to the solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X-100. A cholesterol oxidase and a peroxidase are each dissolved in separate portions of buffer solution and introduced into the buffered solution containing the cholesterol esterase. 4-aminoanti- pyrine is then added to the solution. The resultant solution is a stabilized enzyme solution which can be used in the total cholesterol assay of a serum sample. The stabilized solution can be used in combination with a chromogen diluent solution which is made by dissolving pnenol and TRITON-X-100 in water or a buffer solution. The combination of the stabilized enzyme solution and the chromogen diluent solu- tion provides a solution which has utility in the spectro- photometric assay of total cholesterol.
357900
Hirons & Rogers
Modrovich Ivan E.
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