Enzymatic method for producing bioactive,...

Details Enzymatic method for producing bioactive,... Enzymatic method for producing bioactive,...

C - Chemistry, Metallurgy

12

P

C12P 3/00 (2006.01) C12P 9/00 (2006.01)

Patent

CA 2565121

The invention relates to a method for producing bioactive surfaces by enzymatic modification of molecules or molecular aggregates, in particular, collagen, on surfaces of glass, metals, metallic oxides, plastics, biopolymers or other materials with an amorphous silicon dioxide (silica) or silicones in the cell culture, by tissue engineering or in medical implants, whereby a polypeptide is used for enzymatic modification, which contains a silicatein .alpha. or silicatein .beta. domain. The inventive method promotes the growth, activity and/or mineralisation of cells/cell cultures.

L'invention concerne un procédé de production de surfaces bioactives par modification enzymatique de molécules ou d'agrégats moléculaires, en particulier de collagène, sur des surfaces de verre, de métaux, d'oxydes métalliques, de plastiques, de biopolymères ou d'autres matériaux à dioxyde de silicium amorphe (silice) ou silicones en culture cellulaire, en génie tissulaire ou dans des implants médicaux. Selon ledit procédé, un polypeptide contenant un domaine de silicatéine .beta. ou .alpha. est utilisé pour la modification enzymatique. Le procédé selon l'invention favorise la croissance, l'activité et/ou la minéralisation de cellules/cultures cellulaires.

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