C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/42 (2006.01)
Patent
CA 2634042
A method for measuring sphingomyelin and phosphatidylcholine comprising incubating sphingomyelin and phosphatidylcholine with bacterial sphingomyelinase and bacterial phospholipase D, alkaline phosphatase, choline oxidase, peroxidase, N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline, and 4- aminoantipyrine, preferably for about 45 minutes. A blue dye is generated.
L'invention concerne un procédé permettant de mesurer la sphingomyéline et la phosphatidylcholine consistant à incuber la sphingomyéline et la phosphatidylcholine avec de la sphingomyélinase bactérienne et de la phospholipase D bactérienne, de la phosphatase alcaline, de la choline oxydase, de la peroxydase, de la N-éthyl-N-(2-hydroxy-3-sulfopropyl)-3,5-diméthoxyaniline et de la 4-aminoantipyrine, de préférence pendant au moins 45 minutes. Un colorant bleu est ainsi généré.
Hojjati Mohammad Reza
Jiang Xian-Cheng
Smart & Biggar
The Research Foundation Of State University Of New York
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