Enzymatic oil-degumming method

Details Enzymatic oil-degumming method Enzymatic oil-degumming method

C - Chemistry, Metallurgy

11

B

C11B 3/00 (2006.01) C07F 9/10 (2006.01) C11C 3/00 (2006.01)

Patent

CA 2571694

A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.

Procédé de démucilagination enzymatique d'huiles comestibles consistant à traiter l'huile comestible avec une acyltransférase lipide de manière à transférer un groupe acyle depuis une partie principale du phospholipide vers un ou plusieurs accepteurs d'acide, l'accepteur d'acyle étant un composé quelconque comprenant un groupement hydroxy. Dans un mode de réalisation, l'accepteur d'acyle est de préférence de l'eau et, dans un autre mode de réalisation, l'accepteur d'acyle est de préférence un ou plusieurs stérols et/ou stanols. Lorsque l'accepteur d'acyle est un stanol et/ou un stérol, un ou plusieurs esters stérol et/ou esters stanol sont obtenus. L'acyltransférase lipide comporte une ou plusieurs séquences d'acides aminés telles que: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, ou SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 ou une séquence d'acides aminés comportant au moins 75 % de son identité. Nouvelle acyltransférase lipide comprenant la séquence d'acides aminés illustrée comme SEQ ID No. 16.

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