Enzymatic preparation of glucose syrup from starch

Details Enzymatic preparation of glucose syrup from starch Enzymatic preparation of glucose syrup from starch

C - Chemistry, Metallurgy

12

P

C12P 19/14 (2006.01) C12N 9/28 (2006.01) C13K 1/06 (2006.01)

Patent

CA 2323068

The present invention relates to a process for the preparation of a glucose syrup wherein starch is treated with a Termamyl-like .alpha.-amylase comprising a substitution in Val54 shown in SEQ ID NO: 2 or in the corresponding position in another Termamyl-like .alpha.-amylase. The invention also relates to a glucose syrup obtainable by the process of the invention and the use thereof as ingredient in food products. An object of the invention is also to provide for the use of a Termamyl-like .alpha.-amylase with a substitution in position Val54 using SEQ ID NO: 2 as the backbone or a corresponding position in another Termamyl-like .alpha.-amylase for preparing glucose syrup.

La présente invention concerne un procédé de préparation d'un sirop de glucose dans lequel l'amidon est traité avec une .alpha.-amylase de type Termamyl présentant une substitution en Val54 illustrée dans la SEQ ID No 2, ou dans la position correspondante dans une autre .alpha.-amylase de type Termamyl. L'invention concerne également un sirop de glucose pouvant être obtenu par le procédé de l'invention et son utilisation en tant qu'ingrédient dans des produits alimentaires. L'invention concerne en outre l'utilisation d'une .alpha.-amylase de type Termamyl avec une substitution en position Val54 utilisant la SEQ ID No 2 comme squelette ou une position correspondante dans une autre .alpha.-amylase de type Termamyl pour préparer du sirop de glucose.

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