Enzymatic preparation of phospholipids in aqueous media

C - Chemistry – Metallurgy – 12 – N

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C12N 9/16 (2006.01) C12N 9/18 (2006.01) C12P 9/00 (2006.01) C12P 13/00 (2006.01)

Patent

CA 2376604

Methods of conducting phospholipase-catalyzed transesterification or hydrolysis of phospholipids present in aqueous liposomal suspensions, in the absence of detergents and organic solvents, are described. The method, which employs a water/solid particle interface, gives high conversions, particularly when the solid particle is silica gel having a small particle size and is present at a level at least four times the weight of the reacting lipid. The reaction is useful for the preparation of a variety of differently substituted phospholipids, as well as diacyl glycerols and ceramides.

La présente invention concerne des procédés permettant de réaliser une transestérification à catalyse phospholipase ou une hydrolyse de phospholipides présents dans des suspensions liposomales aqueuses, en absence de détergents et de solvants organiques. Le procédé qui fait intervenir une interface eau/solide particulaire, permet d'obtenir un taux élevé de conversions, particulièrement lorsque le solide particulaire est du silicagel ayant des particules de faible taille et est présent en proportion pondérale au moins quatre fois supérieure à celle du lipide réactif. La réaction convient à la préparation d'un ensemble de phospholipides différemment substitués, ainsi que de diacyl glycérols et céramides.

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