Enzymatic process for the determination of .beta.-lactam...

Details Enzymatic process for the determination of .beta.-lactam... Enzymatic process for the determination of .beta.-lactam...

C - Chemistry, Metallurgy

12

Q

150/15, 150/3.3

C12Q 1/34 (2006.01) C12N 11/08 (2006.01)

Patent

CA 1185881

A B S T R A C T An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyse it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtaind the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.

420214

Affiliated with

Also associated with

Rating

Enzymatic process for the determination of .beta.-lactam... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic process for the determination of .beta.-lactam..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic process for the determination of .beta.-lactam... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1207704