Enzymatic purification process

Details Enzymatic purification process Enzymatic purification process

C - Chemistry, Metallurgy

12

S

195/71

C12S 3/20 (2006.01) C07K 1/36 (2006.01)

Patent

CA 1334948

Controlled enzymatic treatment may be used to selectively degrade undesirable contaminants to a size or charge range which can be more readily removed by subsequent separation steps. Treatment is especially useful for purifying rDNA or monoclonal antibody culture products by using nuclease enzyme treatment to degrade undesirable residual nucleic acids to a molecular size or charge range sufficiently different from the product to be purified so that this difference can be exploited in a subsequent purification step (e.g. precipitation, size exclusion chromatography or ion exchange chromatography).

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