Enzymatic reagent system for total cholesterol assay using...

C - Chemistry – Metallurgy – 12 – Q

Patent

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150/15, 150/3.3

C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 1096282

417D-132A Canada Abstract of the Disclosure A novel enzymatic reagent system is provided for assay- ing for total cholesterol in a liquid containing cholesterol, cho- lesterol esters, oxygen and substances which appear to inhibit the reactions between the enzymes and cholesterol and/or cholesterol esters. The novel reagent system comprises an aqueous solution of a buffering agent, cholesterol oxidase, cholesterol esterase, and a cationic surfactant in an amount sufficient to neutralize the effect of the inhibiting agents.

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