C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 1/06 (2006.01) C12N 1/08 (2006.01) C12S 3/20 (2006.01)
Patent
CA 2137728
The present invention relates to a method for removal of contaminating nucleic acid from a biological sample by exposing the sample to a unique combination of enzymes. The combination of enzymes includes an enzyme which cleaves nucleic acid in an endonuclitic manner and an enzyme which cleaves nucleic acid in an exonuclitic manner. After exposure of the sample to the combination of enzymes, any nucleic acid to be detected may be amplified prior to detection.
L'invention porte sur une méthode pour éliminer les acides nucléiques contaminants dans un échantillon biologique, consistant à exposer l'échantillon à une combinaison donnée d'enzymes. La combinaison d'enzymes comprend une enzyme qui coupe l'acide nucléique de façon endonuclitique, et une enzyme qui le coupe de façon exonuclitique. Après exposition de l'échantillon à la combinaison d'enzymes, tout acide nucléique à déceler peut être amplifié avant la détection.
Keating William E.
Walker G. Terrance
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
LandOfFree
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