C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C07K 17/02 (2006.01) C12P 1/00 (2006.01) C12P 7/62 (2006.01) C12P 7/64 (2006.01) C12P 9/00 (2006.01) C12P 13/00 (2006.01) C12P 19/00 (2006.01)
Patent
CA 2101733
The invention relates to methods for he production of amphiphilic pro- ducts such as esters, sugar-esters, pep- tide-esters, glycolipids, glycoproteins, lipoproteins, peptides, and phosphates of alcohols, sugars, and nucleosides. The methods promote enzymatically catalyzed reactions between hydrophil- ic substrates such as glucerol, glucose, amino acids, and nucleosides, and sec- ond substrates such as free fatty acids, triglycerides, vinylesters, amino acids, and phosphates. The method is also applied to enzymatic reactions with saccharides and polyalcohols. The hy- drophilic substrates are adsorbed to finely divided solid supports such as si- lica gel, diatomaceous earths, or acti- vated chracoals in order to promote the dispersion of the hydrophilic substrates within hydrophobic substrates and solvents. Hydrophobic solvents such as n-hexane and t-butylmethylether may be included in the reaction mixtures. Reactions are conducted under non-aqueous conditions in order to promote reverse hydrolysis. Methods are provided for the production of isomerically pure 1,3-diglycerides. Further methods are disclosed for the production and specific precipitation of pure 1- monogycerides through the use of a reactor/separator system. Enzymes used in the methods include lipases from M. mihei and P. fluorescens, glycosidases such as .beta. - galactosidase, proteases such as chymotrypsin, and acid or alkaline phosphatases. Compositions are provided comprising alcohols, carbohydrates, amino acids, or peptides adsorbed onto solid supports such as silica gel.
Berger Matthias
Laumen Kurt E.
Schneider Manfred P.
Berger Matthias
Gowling Lafleur Henderson Llp
Laumen Kurt E.
Schneider Manfred P.
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