Enzymatic synthesis of oligonucleotides

Details Enzymatic synthesis of oligonucleotides Enzymatic synthesis of oligonucleotides

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) A61K 31/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2069096

2069096 9205287 PCTABS00011 The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.

Affiliated with

Also associated with

Rating

Enzymatic synthesis of oligonucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic synthesis of oligonucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic synthesis of oligonucleotides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1633504