Enzymatic urea assay

C - Chemistry – Metallurgy – 12 – Q

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150/15.1, 150/3.

C12Q 1/58 (2006.01)

Patent

CA 1220122

ABSTRACT OF THE DISCLOSURE A composition, device and method useful as an enzymatic urea assay based on the use of urea amido- lyase comprising urea amidolyase, pyruvate kinase, pyruvate oxidase, mono and divalent cations, phospho- enolpyruvate, thyamine pyrophosphate, ATP, bicar- bonate, phosphate, a color indicator system and op- tionally inorganic phosphate, a buffer having a pH range of from about 6.5 to 9.5, and sodium or potas- sium ferrocyanide. After contacting the test solution or body fluid sample with the assay composition, reading can be accomplished visually or instrument- ally.

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