Enzyme activation protease assay

Details Enzyme activation protease assay Enzyme activation protease assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2474130

A protein reagent is provided for measuring protease enzyme activity in a sample. The protein reagent comprises two inhibition entities joined by a linker comprised of an indicator enzyme donor and an amino acid sequence susceptible to enzymatic cleavage. The protein reagent is substantially inhibited from binding to the cognate enzyme acceptor fragment, while the product of the enzymatic cleavage binds to the cognate enzyme acceptor fragment to form a functional indicator enzyme. The indicator enzyme activity is related to the protease enzyme activity of the sample.

L'invention concerne un réactif protéique servant à mesurer l'activité des protéases dans un échantillon. Ce réactif protéique comprend deux entités inhibitrices unies par un liant qui contient un donneur d'enzyme indicateur et une séquence d'acides aminés sensible à la coupure enzymatique. Ce réactif protéique ne peut pratiquement pas de se lier au fragment de l'accepteur d'enzyme parent, alors que le produit de la coupure enzymatique se lie au fragment de l'accepteur d'enzyme correspondant pour former une enzyme indicateur fonctionnelle. L'activité de l'enzyme indicateur dépend de celle de la protéase dans l'échantillon.

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