Enzyme activity determination method

Details Enzyme activity determination method Enzyme activity determination method

C - Chemistry, Metallurgy

12

Q

150/9, 150/15.2

C12Q 1/34 (2006.01) C12Q 1/44 (2006.01) C12Q 1/54 (2006.01)

Patent

CA 1338167

Method in demostrating the presence of enzyme activity with the aid of a fluorogenic substrate (A) which is converted by the enzyme to a product (B) differing from (A) in respect of its fluorescent properties. The characteristic featur resides in that (A) and/or (B) carries a chromophore which is a triplet sensitizer having a triplet energy level above the excitation level of a lanthanide ion selected from among Eu3+, Tb3+, Dy3+ amd Sm3+ and which is capable of chelating said lanthanide ion by means of an oxygen and/or nitrogen atom in said chromophore, optionally together with chelating groups bound to said chromophore; the said enzymatic conversion from (A) to (B) resulting in that the said chelating capacity is either caused to disappear or created and/or the chromo- phore structure is caused to undergo a change; and measure- ment of fluorescence is performed in the presence of said lanthanide ion by means of time resolved flourescence spectrometry.

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