Enzyme activity screen with direct substrate reloading

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/25 (2006.01) C12N 9/00 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2338772

A method for in vitro selection, from a library of catalyst molecules, of a catalyst molecule of interest having a relatively more efficient specific catalytic activity of interest, as compared to the rest of the catalyst molecules within said library, and wherein said in vitro selection method is characterised by that it allows multiple catalytic activity turn-overs (i.e. substrate to product catalytic activity turn-overs), by the catalyst molecule of interest, before it is finally collected. The method is based on using one or more reagent(s) which are capable of converting a product generated by a catalyst molecule of interest back into the substrate for said catalyst of interest.

La présente invention concerne un procédé de sélection in vitro, à partir d'une bibliothèque de molécule catalytiques, d'une molécule catalytique d'intérêt ayant une activité catalytique d'intérêt spécifique plus efficace, par rapport aux autres molécules catalytiques au sein de la bibliothèque. Ledit procédé de sélection in vitro est caractérisé en ce qu'il permet de nombreux renouvellements de l'activité catalytique (c'est à dire du substrat pour le renouvellement de la production de l'activité catalytique), par la molécule catalytique d'intérêt, avant que celle-ci ne soit éventuellement recueillie. Le procédé est basé sur l'utilisation d'un ou plusieurs réactif(s) capable de reconvertir un produit généré par la molécule catalytique d'intérêt dans le substrat pour ledit catalyseur d'intérêt.

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