Enzyme activity screen with substrate replacement

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/25 (2006.01) C12N 9/00 (2006.01) C12N 9/20 (2006.01) C12N 9/22 (2006.01) C12N 9/42 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12Q 1/00 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2333490

A method for in vitro selection, from a library of catalyst molecules, of a catalyst molecule of interest having a relatively more efficient specific catalytic activity of interest, as compared to the rest of the catalyst molecules within said library, and wherein said in vitro selection method is characterised by that it allows multiple catalytic activity turn-overs (i.e. substrate to product catalytic activity turn-overs), by the catalyst molecule of interest, before it is finally collected.

L'invention concerne un procédé pour la sélection in vitro dans une banque de molécules catalytiques d'une molécule catalytique d'intérêt ayant une activité catalytique spécifique relativement plus efficace par rapport au reste des molécules catalytiques contenues dans cette banque. Ce procédé de sélection in vitro est caractérisé en ce qu'il permet de multiples cycles de reconstitution de l'activité catalytique (c'est-à-dire de multiples cycles de reconstitution de l'activité catalytique pour la réaction substrat-produit) effectués par la molécule catalytique d'intérêt avant qu'elle ne soit finalement recueillie.

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