Enzyme aided removal of color from wood pulps

Details Enzyme aided removal of color from wood pulps Enzyme aided removal of color from wood pulps

D - Textiles, Paper

21

C

D21C 9/10 (2006.01) D21C 5/00 (2006.01) D21C 9/00 (2006.01)

Patent

CA 2285244

Efficient methods of removing color from wood pulp are disclosed. In a preferred embodiment, the method includes the steps of preparing a wood pulp; treating the wood pulp with a cellulase, preferably a cellulase with optimum pH 3-0 - 7.0, and/or solvent, preferably methylamine, to modulate the pulp- fiber-pore-structure; and treating the wood pulp with xylanase wherein the xylanase is capable of releasing chromophores from the pulp, and extracting the wood pulp to remove chromophores. The xylanase preferably is isolated from Bacillus stearothermophilus (ATCC 55696) with molecular weight of approximately 39 kD as determined by SDS-gel electrophoresis, pH optima of pH 6.5 to 10.5, and temperature optima of between 40 ~C and 75 ~C; or alternately, with optimal growth at pH 5.0 to 11.0 and 40 ~C to 75 ~C.

L'invention porte sur des procédés efficaces de décoloration de la pâte de bois. Dans l'exécution préférée, le procédé comprend les étapes suivantes: préparation de la pâte; traitement de la pâte à l'aide d'une cellulase, de préférence une cellulase d'un pH optimal compris entre 3,0 et 7, 0 et/ou un solvant de préférence de la méthylamine, pour moduler la structure pâte-fibres-pores; traitement de la pâte par de la xylanase, laquelle est capable de libérer des chromophores de la pâte; et extraction de la pâte pour éliminer les chromophores. La xylanase est de préférence isolée à partir du Bacillus stearothermophilus (ATCC 55696) d'un poids moléculaire d'environ 39kD mesuré par électrophorèse sur gel SDS, d'un pH optimal compris entre 6,5 et 10.5 et une température optimale comprise entre 40 ·C et 75 ·C, et en alternative, avec une croissance optimale pour un pH entre 5,0 et 11 et une température optimale comprise entre 40 ·C et 75 ·C.

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