Enzyme amplified lanthanide chelate luminescence assay

Details Enzyme amplified lanthanide chelate luminescence assay Enzyme amplified lanthanide chelate luminescence assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/25 (2006.01) C12Q 1/26 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2067394

A method and compounds useful for this method are described for enzyme- amplified signal detection in analytical assays requiring extremely high detection sensitivity which uses a substrate capable of being transformed by an enzyme from a com- pound which does not form a luminescent lanthanide chelate into a product which forms a luminescent lanthanide chelate. The method in which a substrate not capable of forming a highly luminescent lanthanide chelate is enzymatically altered to produce a product which forms a highly luminescent lanthanide chelate and hence is particularly useful in time-resolved luminescence anal- ysis as required in many different heterogeneous or homogeneous assay formats is described herein. Above mentioned com- pounds include benzoic acid, 2-phosphonooxy-derivatives (a), benzophenone, 4- phosphonooxy-derivates (.beta.), as well as (~) and (.delta.).

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