Enzyme assay of biochemical substances

Details Enzyme assay of biochemical substances Enzyme assay of biochemical substances

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/26 (2006.01)

Patent

CA 1335170

This invention relates to an enzyme assay for qunatita- tive analysis of biochemical substances using NAD(P)-NAD(P)H system and utilizing hydrogen peroxide, in which isocitric acid, metal ions and isocitrate dehydrogenase are previously added to a test sample, thereby consuming the endogenous substances that interfere the analysis, and at the same time regenerating NAD(P)H reduced. The isocitrate dehydrogenase is then inactivated by addition of a chelating agent, an enzyme or substrate that generates hydrogen peroxide as reaction product is added simultaneously or thereafter, and the amount of hydrogen peroxide thus formed is measured. Biochemical substances that generate hydrogen peroxide as reaction product can be correctly analyzed by this method with no adverse effect of endogenous substances because these interfering substances have been removed prior to measurement.

600565

Affiliated with

Also associated with

Rating

Enzyme assay of biochemical substances does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme assay of biochemical substances, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme assay of biochemical substances will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1335071