Enzyme assays

Details Enzyme assays Enzyme assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01) C09B 7/02 (2006.01) G01N 33/573 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2051144

ABSTRACT OF THE DISCLOSURE Accurate enzyme assays suitable for use in immunoassay techniques are now possible for enzymes associated with the dimerization of indoxyl esters to form the corresponding indigo, the assays utilizing the production of hydrogen peroxide from this reaction, which is found to be sufficiently consistent that it can be used to accurately calculate enzyme activity down to levels of 1 in 1000 compared to colorimetric techniques for the same enzymes.

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