Enzyme-based device for environmental monitoring

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/34 (2006.01) C12Q 1/34 (2006.01) C12Q 1/46 (2006.01)

Patent

CA 2582504

A sensor for the intermittent or continuous detection of the presence of at least one analyte in an environmental sample includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a product compound or (ii) be inhibited by the analyte in the presence of a substrate compound. The sensor also includes at least one sensor for monitoring or at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the analyte and the enzyme. Optionally, each of the enzyme and the indicator compound are incorporated within a single polymer.

L'invention concerne un capteur permettant de détecter de manière intermittente ou continue la présence d'au moins un analyte dans un échantillon prélevé dans l'environnement. Ce capteur comprend au moins une enzyme qui est sélectionnée soit (i) de manière à catalyser une réaction avec l'analyte afin de transformer chimiquement l'analyte en un composé résultant, soit (ii) de manière à être inhibée par l'analyte en présence d'un composé substrat. Ce capteur comprend en outre au moins un capteur permettant de surveiller au moins un composé indicateur choisi pour produire un changement d'état mesurable sous l'effet de l'interaction de l'analyte avec l'enzyme. L'enzyme et le composé indicateur peuvent être éventuellement chacun incorporés dans un polymère unique.

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