Enzyme blends for fermentation

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/30 (2006.01) C12N 9/34 (2006.01) C12N 9/58 (2006.01) C12N 9/62 (2006.01) C12Q 1/40 (2006.01)

Patent

CA 2702949

The present invention relates to an enzyme blend composition comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease. The present invention is further directed to a method for producing end products such as alcohols from fermentable sugars, comprising the steps of: (a) contacting a slurry comprising a milled grain that contains starch with an alpha amylase to produce a liquefact; (b) contacting the liquefact with a glucoamylase, an acid stable alpha amylase, and an acid fungal protease, to produce fermentable sugars; and (c) fermenting the fermentable sugars in the presence of a fermenting organism to produce end products.

La présente invention concerne une composition de mélange d'enzymes comprenant une glucoamylase, une alpha amylase acide stable et une protéase acide fongique. La présente invention concerne également un procédé de production de produits finaux tels que des alcools à partir de sucres fermentescibles, comprenant les étapes consistant: (a) à mettre en contact une suspension épaisse comprenant un grain moulu qui contient de l'amidon avec une alpha amylase afin de produire un produit de liquéfaction; (b) à mettre en contact le produit de liquéfaction avec une glucoamylase, une alpha amylase acide stable. et une protéase acide fongique, afin de produire des sucres fermentescibles; et (c) à faire fermenter les sucres fermentescibles en présence d'un organisme de fermentation afin de produire des produits finaux.

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