G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01) G01N 27/64 (2006.01)
Patent
CA 2471668
A method and tools for the biochemical processing of protein samples towards their analysis and identification by, for example, mass spectrometry (MS). The method involves the reversible (non-covalent) immobilization, purification and concentration of proteins onto a solid surface and subsequent solid-phase enzyme-catalyzed proteolysis of the proteins. The constituent peptides are recovered in near-quantitative yields in a format ideally suited for identification by routine MS technologies. The process is easily adapted such that additional chemical and/or enzymatic transformation(s) of the immobilized proteins or peptides can be performed to facilitate the acquisition of and increase the information content obtained from the MS analysis.
Procédé et outils destinés au traitement biochimique des échantillons de protéines à des fins de leur analyse et de leur identification, par exemple, par spectrométrie de masse. Le procédé comprend l'immobilisation réversible (non covalente), la purification et la concentration des protéines sur une surface solide, suivie de la protéolyse catalysée par enzymes des protéines. Les peptides constitutifs sont récupérées avec des rendements se rapprochant des quantitatifs dans un format idéalement conçu pour l'identification au moyen des technologies courantes de spectrométrie de masse. Le processus peut être facilement adapté de manière à permettre la ou les transformation(s) enzymatique(s) et/ou chimique(s) complémentaire(s) afin de faciliter l'acquisition et l'augmentation du contenu informationnel obtenu au moyen de l'analyse par spectrométrie de masse.
Duewel Henry
Figeys Joseph Michel Daniel
Goh Theo
Lebihan Thierry
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Mds Proteomics Inc.
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