Enzyme chromatographic immunoassay

Details Enzyme chromatographic immunoassay Enzyme chromatographic immunoassay

G - Physics

01

N

150/15, 167/45

G01N 33/558 (2006.01) G01N 33/532 (2006.01)

Patent

CA 1192122

ENZYME CHROMATOGRAPHIC IMMUNOASSAY ABSTRACT OF THE DISCLOSURE Chromatographic immunoassay employing a specific binding pair member and a label conjugate which delineates a border whose distance from one end of the chromatograph relates to the amount of analyte present. By combining the label conjugate and sample in a solution and immunochromato- graphing the solution, or employing a combination of enzymes, one enzyme being the label and the other enzyme affixed to the chromatographic support, the position of the border defined by the label can be related to the amount of analyte in the sample solution. Preferably, an immunochromatograph is employed having both a specific binding pair member and an enzyme affixed to the support. A sample is chromatographed and the amount of analyte is determined by (1) contacting the chromatograph with a second enzyme conjugated with a speci- fic binding pair member which binds to the chromatograph in proportion to the amount of analyte bound to the chromato- graph, or (2) including the second enzyme conjugate with said sample, resulting in a defined border related to the amount of analyte in the sample. The two enzymes are related in that the substrate of one is the product of the other, so that upon contact of the chromatograph with appopriate reagents, a detectable signal develops which permits detection of the border to which the analyte traveled. This distance can be related to the amount of analyte present in the sample.

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