Enzyme compositions and methods for the improved enzymatic...

Details Enzyme compositions and methods for the improved enzymatic... Enzyme compositions and methods for the improved enzymatic...

C - Chemistry, Metallurgy

12

P

C12P 19/14 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01)

Patent

CA 2655640

A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one .beta.- glucosidase enzyme and a binding agent for binding the .beta.-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose.

Procédé pour l'hydrolyse enzymatique de la cellulose permettant de produire un produit d'hydrolyse comprenant du glucose à partir d'une charge d'alimentation lignocellulosique prétraitée et enzymes destinées à être utilisées dans le procédé. Le procédé consiste à hydrolyser partiellement une suspension épaisse aqueuse d'une charge d'alimentation lignocellulosique prétraitée avec des enzymes de type cellulase, une ou plus d'une enzyme de type .beta.-glucosidase et un agent liant servant à fixer l'enzyme de type .beta.-glucosidase aux matières solides fibreuses présentes dans la suspension épaisse aqueuse. Les matières solides fibreuses non hydrolysées sont ensuite séparées de la suspension épaisse aqueuse hydrolysée. Les matières solides fibreuses séparées ainsi obtenues sont ensuite remises en suspension dans une solution aqueuse pour produire une suspension épaisse remise en suspension. On poursuit alors l'hydrolyse pour produire le produit d'hydrolyse comprenant du glucose.

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