Enzyme compositions for the improved enzymatic hydrolysis of...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/14 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01)

Patent

CA 2655645

A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one .beta.-glucosidase enzyme and a binding agent for binding the .beta.-glucosidase enzyme to fiber solids present in the aqueous slurry. During the hydrolysis, both the cellulase enzyme and .beta.-glucosidase enzyme bind to the fiber solids. The hydrolysis is performed in a solids-retaining hydrolysis reactor so that unhydrolyzed fiber solids and bound enzyme are retained in the reactor longer than the aqueous phase of the slurry.

Procédé pour l'hydrolyse enzymatique de la cellulose pour produire un produit d'hydrolyse comprenant du glucose à partir d'une charge d'alimentation lignocellulosique prétraitée et enzymes destinées à être utilisées dans le procédé. Le procédé consiste à hydrolyser une suspension épaisse aqueuse d'une charge d'alimentation lignocellulosique prétraitée avec des enzymes de type cellulases, une ou plus d'une enzyme de type .beta.-glucosidase et un agent liant servant à fixer l'enzyme de type .beta.-glucosidase aux matières solides fibreuses présentes dans la suspension épaisse aqueuse. Au cours de l'hydrolyse, à la fois l'enzyme de type cellulase et l'enzyme de type .beta.-glucosidase se fixent aux matières solides fibreuses. L'hydrolyse est effectuée dans un réacteur d'hydrolyse retenant les matières solides pour que les matières solides fibreuses non hydrolysées et les enzymes liées à celles-ci soient retenues dans le réacteur plus longtemps que la phase aqueuse de la suspension épaisse.

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