Enzyme containing liquid and delivery system for detection...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/25 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12N 9/96 (2006.01) C12Q 1/00 (2006.01) C12Q 1/28 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2660436

An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.

L'invention concerne un capteur à base d'enzyme aqueuse comprenant au moins une enzyme et au moins un composé indicateur permettant d'indiquer une réaction d'au moins une enzyme avec au moins un analyte cible, l'enzyme et l'indicateur pouvant être administrés, de préférence par pulvérisation, sur une surface possédant l'analyte cible sans échantillonnage de la surface. L'invention concerne un capteur à base d'enzyme aqueuse capable de détecter un substrat spécifique à une enzyme comme analyte. Ce capteur à base d'enzyme aqueuse comprend également au moins un substrat spécifique à une enzyme dans lequel le capteur détecte l'inhibition de l'enzyme. Le capteur à base d'enzyme aqueuse comprend éventuellement au moins un additif de dispersion de lumière, un polymère adhésif, un stabilisateur de protéine, un sucre de stabilisation de protéine, un agent tensioactif et un solvant et leurs combinaisons. L'invention concerne également un système de distribution et un procédé de détection d'un produit chimique cible sur une surface sans échantillonnage de la surface.

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