C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/34 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/00 (2006.01) C12Q 1/37 (2006.01) G01N 27/327 (2006.01) G01N 27/416 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2245664
The present invention provides a biosensor for use in detecting the presence of an enzyme or enzymes in a sample. The biosensor comprises a membrane and means for determining the impedance of the membrane. The membrane includes ionophores therein to which are attached linkers. The linkers are cleavable by the enzyme or enzymes to be detected, with the cleavage of the linker causing a change in the ability of ions to pass through the membrane via the ionophores.
La présente invention concerne un biocapteur servant à détecter la présence d'une ou plusieurs enzymes dans un échantillon. Ce biocapteur comprend une membrane et un organe de détermination de l'impédance de la membrane, laquelle comporte des ionophores auxquels sont fixés des segments de liaison. Ces derniers peuvent subir un clivage du fait de l'enzyme, ou des enzymes, qu'il s'agit de détecter. Ce clivage du segment de liaison produit un changement de la capacité des ions à traverser la membrane par l'intermédiaire des ionophores.
Braach-Maksvytis Vijoleta Lucija Bronislava
Cornell Bruce Andrew
Raguse Burkhard
Thomson David Geoffrey
Ambri Limited
Australian Membrane And Biotechnology Research Institute
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
The University Of Sydney
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