Enzyme for cleavage of the anchor region of surface proteins...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/52 (2006.01) C07K 16/40 (2006.01) C12N 9/99 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2201616

An enzyme which cleaves at the sequence LPXTGX (SEQ ID NO:1) of the surface proteins of gram positive bacteria is provided. The enzyme was isolated from a group A Streptococcus. In addition, a method of detecting the enzyme, a method of isolating the enzyme, and methods of inhibiting the enzyme are disclosed. The figure is a schematic illustrating the positioning of the surface protein in the bacterial membrane, and the cleavage step which leads to infective bacteria.

L'invention concerne une enzyme qui effectue un clivage au niveau de la séquence LPXTGX (numéro d'identification: 1) des protéines de surface de bactéries Gram positif. Cette enzyme a été isolée à partir d'un streptocoque du groupe A. L'invention concerne également un procédé permettant de détecter ladite enzyme, un procédé permettant de l'isoler, et des procédés permettant de l'inhiber. La figure montre, de façon schématique, le positionnement de la protéine de surface dans la membrane bactérienne, ainsi que l'étape de clivage qui conduit à l'apparition de bactéries infectieuses.

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