Enzyme fusion proteins and their use

Details Enzyme fusion proteins and their use Enzyme fusion proteins and their use

C - Chemistry, Metallurgy

12

N

C12N 9/42 (2006.01) A23K 1/165 (2006.01) C11D 3/386 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) D06M 16/00 (2006.01)

Patent

CA 2648926

Cellulase fusion proteins comprising an endoglucanase core region and a heterologous cellulose binding domain are described. The fusion proteins may be produced by recombinant techniques using appropriate polynucleotides, expressing vectors and host cells. The fusion proteins and enzyme preparations thereof are useful in treating cellulosic material, such as textile material, and they are particularly useful in biostoning denim or in biofinishing fabrics and garments. In addition the fusion proteins may be used in pulp and paper industry, oil extraction from plants, detergent compositions, or for improving the quality of animal feed.

L'invention concerne des protéines de fusion de cellulase comprenant une zone centrale d'endoglucanase et un domaine de liaison à la cellulose hétérologue. Les protéines de fusion peuvent être produites par des techniques de recombinaison à l'aide de polynucléotides appropriés, exprimant des vecteurs et des cellules hôtes. Les protéines de fusion et les préparations enzymatiques de celles-ci sont utiles dans le traitement de la matière cellulosique, telle que la matière textile, et elles sont particulièrement utiles pour le lavage aux enzymes (biostoning) du denim ou le traitement biologique (biofinishing) des tissus et vêtements. De plus, les protéines de fusion peuvent être utilisées dans l'industrie des pâtes et du papier, l'extraction d'huile végétale, les compositions de détergent ou l'amélioration de la qualité de l'alimentation animale.

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