C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/40 (2006.01) C12N 1/19 (2006.01) C12N 9/00 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/81 (2006.01) C12P 7/62 (2006.01)
Patent
CA 2462942
A process for producing a polyester, which is obtained by the homopolymerization or copolymerization of a 3-hydroxyalkanoic acid and has biodegradability and excellent properties, using a yeast as a host. One or more enzyme genes participating in the synthesis of a polyester, each having DNA encoding peroxisome-targeting signal added thereto, are constructed and an enzyme gene expression cassette is transferred into a yeast. The thus obtained transformant is cultured so as to accumulate a polyester obtained by the homopolymerization or copolymerization of a 3-hydroxyalkanoic acid in the cells. Then, the polymer can be collected from the culture.
La présente invention concerne un procédé de production d'un polyester, lequel est obtenu par homopolymérisation ou copolymérisation d'un acide hydroxyalcanoïque et présente une biodégradabilité et d'excellentes propriétés, à l'aide d'une levure servant de hôte. Un ou plusieurs gènes d'enzyme participant à la synthèse d'un polyester, auxquels on a ajouté un signal de ciblage de peroxisome codant l'ADN, sont construits et une cassette d'expression d'un gène d'enzyme est transférée dans une levure. Le transformant ainsi obtenu est mis en culture par homopolymérisation ou copolymérisation d'un acide 3-hydroxyalcanoïque dans les cellules. Ensuite, ledit polymère peut être récupéré à partir de la culture.
Fukuchi Takeshi
Matsumoto Keiji
Ohta Akinori
Osakada Fumio
Takagi Masamichi
Kaneka Corporation
Riches Mckenzie & Herbert Llp
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