Enzyme-immobilization carrier and preparation thereof

C - Chemistry – Metallurgy – 12 – P

Patent

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195/123, 195/35.

C12P 21/00 (2006.01) C08G 85/00 (2006.01) C12N 11/02 (2006.01) C12N 11/08 (2006.01)

Patent

CA 1107669

ABSTRACT OF THE DISCLOSURE A novel enzyme-immobilization resin and process for its manufac- ture is provided herein. The resin comprises a macroporous, synthetic anion- exchange resin having a specific surface area of at least 1 m2/g-dry resin and containing macropores with a pore size of 100 .ANG. to 2,000 .ANG., of which total volume is at least 0.1 cc/g-dry resin; the resin comprising (a) a phenol-formaldehyde condensate matrix and diethylaminoethyl groups linked to the matrix through an ether linkage, wherein the ion-exchange capacity of the resin is not less than 1 meq/g-dry resin, (b) a phenol-formaldehyde condensate matrix having primary amino groups, secondary amino groups or a mixture thereof as an anion-exchanger and diethylaminoethyl groups linked to the matrix through an ether linkage or to the primary or secondary amino groups to form a secondary or tertiary amino group respectively, wherein the ion-exchange capacity due to the diethylaminoethyl groups is not less than 0.5 meq/g-dry resin and the total ion-exchange capacity is not less than 1 meq/g-dry resin, or (c) a crosslinked polystyrene matrix having pri- mary amino groups, secondary amino groups or a mixture thereof as an anion- exchanger and diethylaminoethyl groups linked to the primary or secondary amino group to form a secondary or tertiary amino group respectively, wherein the ion-exchange capacity due to the diethylaminoethyl groups is not less than 0.5 meq/g-dry resin and the total ion-exchange capacity is not less than 1 meq/g-dry resin. This provides an immobilized enzyme high in activity with good activity retentivity and also high in the amount of the immobilized enzyme per unit weight of the carrier. The resin is provided by reacting the diethylaminoethyl derivative or its salt with granules of the above defined synthetic resin of a macroporous type. The reaction takes place in the presence of an alkaline compound.

297065

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