Enzyme immobilization with pullulan gel

Details Enzyme immobilization with pullulan gel Enzyme immobilization with pullulan gel

C - Chemistry, Metallurgy

12

N

195/35.3

C12N 11/02 (2006.01) C08B 37/00 (2006.01) C12N 11/10 (2006.01)

Patent

CA 1093991

ABSTRACT OF THE DISCLOSURE Enzymes are immobilized by a process wherein the carrier used for immobilizing is a hydrophilic pullulan gel having a three- dimensionally reticulated structure obtained by the reaction between pullulan and a bifunctional compound capable of forming an ether linkage with the hydroxyl group contained in the glucose unit of pullulan, or an ionic pullulan gel obtained by the reaction between said hydrophilic pullulan gel and a compound having at one end an ionizable group and at the other end a functional group capable of forming an ether linkage with the hydroxyl group in the pullulan gel in the presence of an alkaline compound. Immobilized enzymes prepared according to this process have a high activity and good retention of the activity.

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