Enzyme immunoassay

G - Physics – 01 – N

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G01N 33/573 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1215322

ABSTRACT The present invention is characterized in that, in the conventional solid-phase enzyme immunoassay, all the steps of the immunoassay from the step of immunological reaction of the immunologically reactive component(s) or an immunological complex thereof with a carrier-adsorbed reactant through the step of measuring the activity of enzyme contained in the resulting insolubilized product ,are carried out by applying chromatography with a single column. The method of the present invention has the advantages in that a variety of antigens or antibodies can be assayed by using an identical enzyme or insoluble carrier, and that high sensitivity and accuracy can be attained without difficult operations for the method.

412425

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