C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
167/45
C12Q 1/00 (2006.01) G01N 33/53 (2006.01)
Patent
CA 1140046
ABSTRACT OF THE DISCLOSURE A method of quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample to provide a 2'-O-acyl derivative of said cyclic nucleotide; causing said acyl-cyclic nucleotide and a specific quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide, corresponding to the cyclic nucleotide to be assayed, and a enzyme through a dicarboxylic acid to undergo competitive reaction with a specific quantity of an antibody with respect to a corresponding cyclic nucleotide; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and determinine the quantity of the cyclic nucleotide by measuring the enzyme activity of either of the enzyme-labeled cyclic nucleotides. For this analysis, a reagent comprising an enzyme-labeled cyclic nucleotide is used.
352143
Macrae & Co.
Yamasa Shoyu Kabushiki Kaisha
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