Enzyme of use in chitosan hydrolysis

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/76 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2085292

The present invention relates to a naturally occuring, isolated and characterized microorganism producing hydrolase enzyme, specifically, chitosanase; chitosanase isolated thereof; the nucleic acid sequence of the portion of the gene encoding this chitosanase; complete amino acid sequence of this chitosanase; hybrid plasmids containing the related gene; host microorganisms transformed with the plasmids; recombinant microorganisms overexpressing the enzyme; and to enzymatic treatment of chitosan with the aforementioned enzyme resulting in molecular weight decrease, viscosity decrease and increase of solubility of chitosan in water.

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