Enzyme paper for the indication of cholinesterase-...

C - Chemistry – Metallurgy – 12 – Q

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150/15.2, 150/3.

C12Q 1/46 (2006.01) C12N 11/12 (2006.01)

Patent

CA 1259553

ABSTRACT The invention relates to an enzyme paper, which is suit- able for use in the detection of cholinesterase-inhibitors in a gas or liquid, a method of using the enzyme paper for such detec- tion and, a method of making such a paper,which method involves an advantageous immobilization and stabilization of the enzyme on the paper. The enzyme paper consists of an enzyme carrier being a type of chromatography paper with ion exchange capacity and, having immobilized on the carrier, a cholinesterase enzyme, preferably from plaice, at least one component stabilizing the cholinesterase enzyme, preferably a carbohydrate, e.g. sucrose and dextran, and a surface-active substance, e.g. Tween 80*. *Trade mark

491908

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