C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/39, 195/134.
C12N 15/57 (2006.01) C07K 1/107 (2006.01) C12N 9/02 (2006.01) C12N 9/50 (2006.01) C12P 13/04 (2006.01) C12Q 1/30 (2006.01) C12Q 1/37 (2006.01)
Patent
CA 2039174
- 77 - ENZYME PARTICIPATING IN C-TERMINAL AMIDATION, PROCESS FOR PREPARING AND USE OF THE SAME ABSTRACT OF THE DISCLOSURE An enzyme participating in C-terminal amidation which acts on a peptide C-terminal glycine adduct represented by the following formula (I): Image (I) (wherein A represents a residue other than .alpha.-amino group or imino group and .alpha.-carboxylic group derived from naturally occurring .alpha.-amino acid, X represents hydrogen atom or a residue of an amino acid derivative which is bonded to N atom through carbonyl group) to form a peptide C-terminal .alpha.-hydroxylglycine adduct represented by the following formula (II): Image (II) (wherein A and X have the same meanings as above, and an enzyme participating in peptide C-terminal amidation of a peptide terminal glycine adduct which acts on a peptide C-terminal .alpha.-hydroxylglycine adduct represented by the above formula (II) to form a C-terminal amidated compound represented by the following formula (III): Image (III) (wherein A and X have the same meanings as described above) a method of producing the same, and the use thereof.
Fuse Yuka
Ifuku Ohji
Iida Toshii
Kaminuma Toshihiko
Kato Ichiro
Borden Ladner Gervais Llp
Shiseido Company Ltd.
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