Enzyme preparation containing thermostable dna polymerase,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2749693

Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.

L'invention concerne une préparation d'ADN polymérase thermostable permettant de réduire de façon illimité le risque de résultats faux-positifs dans la détection d'un analyte de micro-organisme par une réaction d'amplification génique, et permettant ainsi l'amplification sélective d'ADN pour détecter l'analyte de micro-organisme, même lorsque la quantité d'analyte est faible et donc que la quantité d'ADN prélevé sur l'analyte de micro-organisme est extrêmement faible et peut être produite à un coût réduit. L'invention concerne également un procédé de quantification ou quantification/identification d'un organisme d'intérêt de façon rapide, pratique et avec une sensibilité élevée, au moyen de cette préparation.

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