Enzyme production and purification

Details Enzyme production and purification Enzyme production and purification

C - Chemistry, Metallurgy

12

N

195/123, 195/33.

C12N 9/10 (2006.01) C12P 19/04 (2006.01) C12P 19/08 (2006.01)

Patent

CA 1178546

ABSTRACT There is described a process for the production of a polysaccharide-producing enzyme, which comprises ferment- ing an appropriate enzyme producing organism in aqueous sucrose, characterised in that the sucrose concentration is maintained at between 1 and 10g/1 throughout all but the last part of the fermentation and that the dissolved oxygen content of the fermentation is controlled. There is also described a method for the purification of a polysaccharide producing enzyme, which comprises absorbing a mixture containing the enzyme on an anionic resin and removing the enzyme from the resin with a sugar containing eluant, and a novel highly purified form of polysaccharide producing enzyme.

381094

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