C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 1/21 (2006.01) C12N 9/28 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2376621
The invention relates to the production by recombinant technology of enzymes having desired activity. The invention provides methods employing display vehicle technology allowing to select enzymes having desired catalytic activity. The invention provides a method for selecting an enzyme mutant with desired catalytic activity comprising displaying each of a multitude of mutants of said enzyme on the surface of a display vehicle comprising a nucleic acid encoding said mutant and selecting for a display vehicle carrying nucleic acid encoding an enzyme mutant having at least a site other than its catalytic site mutated.
L'invention concerne la production d'enzymes, possédant une activité désirée, à l'aide de technologies de recombinaison. Cette invention fournit des techniques qui utilisent une technologie à support d'affichage permettant de sélectionner des enzymes possédant une activité catalytique désirée. Elle fournit également une technique permettant de sélectionner un mutant d'enzyme possédant une activité catalytique désirée, qui consiste à afficher chaque mutant dudit enzyme à la surface d'un support d'affichage comprenant un acide nucléique codant pour ledit mutant, et à sélectionner un support d'affichage comprenant un acide nucléique codant pour un mutant d'enzyme possédant au moins un site autre que son site catalytique muté.
Aehle Wolfgang
Beekwilder Martinus Julius
Quax Wilhelmus Johannes
Verhaert Raymond Michael Dimphena
Borden Ladner Gervais Llp
Rijksuniversiteit Groningen
LandOfFree
Enzyme selection does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzyme selection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme selection will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1500665