Enzyme stabilization by cationic surfactants

Details Enzyme stabilization by cationic surfactants Enzyme stabilization by cationic surfactants

C - Chemistry, Metallurgy

12

N

C12N 9/96 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2335153

The present invention provides methods and compositions for protein stabilization, particularly the stabilization of polymerases in aqueous solutions with cationic surfactants. The present invention further provides cationic surfactants, including polyethoxylated amines, that stabilize thermostable and thermolabile enzymes in solution. These surfactants stabilize the activity of various enzymes, including thermostable DNA polymerases, thermolabile DNA polymerases and reverse transcriptases.

Cette invention, qui a trait à des techniques ainsi qu'aux compositions afférentes visant à la stabilisation de protéines, notamment à la stabilisation de polymérases dans des solutions aqueuses et ce, au moyen de tensioactifs cationiques, concerne également des tensioactifs cationiques, au nombre desquels se trouvent des amines polyéthoxylées à même de stabiliser des enzymes thermostables et thermolabiles en solution. Ces tensioactifs stabilisent l'activité de diverses enzymes, celle, notamment, des ADN polymérases et des transcriptases inverses.

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