Enzymes and formulations for broad-specificity...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/57 (2006.01) A62D 3/02 (2007.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01K 67/027 (2006.01) B01F 17/30 (2006.01) C02F 3/00 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C07K 19/00 (2006.01) C09D 5/00 (2006.01) C12N 5/16 (2006.01) C12N 9/02 (2006.01) C12N 9/08 (2006.01) C12N 9/14 (2006.01) C12N 9/16 (2006.01) C12N 9/48 (2006.01) C12N 11/00 (2006.01) C12N 15/11 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/55 (2006.01) C12N 15/67 (2006.01) C12N 15/82 (2006.01) C12P 1/00 (2006.01

Patent

CA 2602185

The invention provides novel enzymes and methods for enzyme-based active decontamination, e.g., nerve agent detoxification. In one aspect, the invention provides enzymes having non-heme chloroperoxidase (nhCPOs) activity, and methods for bleaching and degradation of lignin. The invention provides polypeptides, nucleic acids, infective vehicles, transduced or infected cells or plants and/or transgenic plants to, and methods of using them, e.g., provide self-protecting, pesticide-resistant and/or herbicide-resistant cells or plants, where in one aspect the polypeptides of the invention act to hydrolyzing P-S or P-F bonds and detoxify acetylcholinesterases or butyrylcholinesterases. In one aspect, the invention provides an enzyme mixture or composition that provides broad- spectrum, agent-specific oxidation and hydrolysis activities as well as hypohalite generation for oxidation or hydrolysis of V-agents, G-agents and H-agents and which generates oxidants such as chlorine dioxide and reactive radicals for killing of biological agents, including anthrax spores.

L'invention concerne de nouvelles enzymes et de nouveaux procédés de décontamination active à base d'enzymes, par exemple la désintoxication d'agents neurotoxiques. Dans un aspect, l'invention concerne des enzymes possédant une activité chloroperoxydase non hème (nhCPO) et des procédés de blanchiment et de dégradation de lignine. Cette invention concerne des polypeptides, des acides nucléiques, des véhicules infectieux, des cellules ou des plantes infectées ou transduites, et/ou des plantes transgéniques et des procédés utilisant celles-ci afin d'obtenir par exemple des plantes ou des cellules résistant aux herbicides et/ou aux pesticides, à protection autonome. Dans un aspect, les polypeptides de l'invention agissent de façon à hydrolyser des liaisons P-S ou P-F et désintoxiquer des acétylcholinestérases ou des butyrylcholinestérases. Dans un aspect, l'invention concerne un mélange d'enzymes ou une composition qui comprend des activités d'oxydation ou d'hydrolyse spécifique de l'agent à large spectre ainsi qu'une génération d'hypohalogénite pour l'oxydation ou l'hydrolyse d'agents V, d'agents G et d'agents H et qui génère des oxydants tels que la dioxine de chlorure et des radicaux réactifs destinés à tuer des agents biologiques, notamment des spores d'anthrax.

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