C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/125, 195/139
C12N 9/38 (2006.01) C12N 11/04 (2006.01) C12N 11/10 (2006.01) C12P 19/02 (2006.01)
Patent
CA 1133410
ABSTRACT A novel .beta.-galactosidase comprises an enzyme having a thermal stability such that when in purified form it has an active half life of at least 1 1/2 hours at 55°, at least 1 hour at 60°C and at least 10 minutes at 65°C, as measured using ONPG as substrate, said enzyme being derived from a bacterium of species Bacillus stearothermophilus. There is also provided a process for the production of the novel .beta.-galactosidase, comprising culturing in a medium containing appropriate nutrients a micro-organism of species B. stearothermophilus having characteristics similar to or the same as the strains identified as NCIB 11407-11413, and which contains the .beta.-galactosidase of the invention. Additionally there is provided a process for the hydrolysis of lactose to a product comprising glucose and galactose which comprises contacting lactose with the .beta.-galactosidase of the invention e.g. at a temperature of at least 55°C preferably at least 60°C or especially about 65°C.
329181
Griffiths Mansel W.
Muir David D.
Phillips John D.
Fetherstonhaugh & Co.
National Research Development Corporation
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