Enzymes and their use in drug detection

Details Enzymes and their use in drug detection Enzymes and their use in drug detection

C - Chemistry, Metallurgy

12

N

C12N 9/18 (2006.01) C12M 1/40 (2006.01) C12N 1/20 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12P 17/18 (2006.01) C12Q 1/00 (2006.01) C12Q 1/32 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2055442

Two enzymes, one an acetylmorphine carboxyesterase (AMCE), the other a morphine dehydrogenase (MDH), have been isolated from bacteria. The AMCE degrades heroin to morphine and the MDH oxidises morphine to morphinone, with the aid of a cofactor. These reactions are used in detection of heroin (using the two reactions coupled together) or morphine. The enzymes can be incorporated in sensors for this purpose.

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