C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/78 (2006.01) C12S 99/00 (2010.01) C12N 1/20 (2006.01) C12N 11/04 (2006.01) C12N 11/08 (2006.01) C12P 7/40 (2006.01) C12Q 1/34 (2006.01)
Patent
CA 2238443
New nitrilase enzymes are provided which have Km at pH 7.0 for acrylonitrile of 500 µM or below. New enzymes also have Ki at pH 7.0 for ammonium acrylate of at least 100 mM. In particular, new nitrilases have a value of the ratio of the said Ki to the said Km of at least 200. Particularly preferred nitrilases are obtainable from the microorganisms Rhodococcus rhodochrous NCIMB 40757 or NCIMB 40833. These nitrilases can be used in processes of converting acrylonitrile to ammonium acrylate in aqueous or vapour form and for detecting low levels of nitrile in aqueous or vapour form.
L'invention porte sur de nouvelles enzymes du genre nitrilases, dont le Km à un pH de 7,0 pour les acrylonitriles est de 500 $(m)M ou moins, et le Ki à un pH de 7,0 pour l'acrylate d'ammonium est d'au moins 100mM. Elles présentent en outre un rapport Ki/Km d'au moins 200. Les nitrilases particulièrement préférées s'obtiennent à partir des micro-organismes Rhodococcus rhodochrous NCIMB 40727 ou NCIMB 40833. Ces nitrilases peuvent servir à la conversion de l'acrylonitrile en acrylate d'ammonium sous forme aqueuse ou de gazeuse, et à la détection de faibles niveaux de nitriles sous forme aqueuse ou gazeuse.
Armitage Yvonne Christine
Hughes Jonathan
Webster Neil Andrew
Allied Colloids Limited
Borden Ladner Gervais Llp
Ciba Specialty Chemicals Water Treatments Limited
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