Enzymic method of detecting analytes and novel substrates...

Details Enzymic method of detecting analytes and novel substrates... Enzymic method of detecting analytes and novel substrates...

C - Chemistry, Metallurgy

07

H

150/15, 167/45,

C07H 19/04 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1258673

ABSTRACT OF THE DISCLOSURE ENZYMIC METHOD OF DETECTING ANALYTES AND NOVEL SUBSTRATES THEREFOR A small enzymically inactive peptide fragment of an enzyme (e.g. ribonuclease S-peptide) is used as the label and conjugated with the complementary fragment (S-protein) to form an enzyme which catalyses a primary reaction whose product is, or leads to, an essential coenzyme or prosthetic group for a second enzyme which catalyses a secondary reaction leading to a detectable result indicating the presence of analyte. Also disclosed are novel synthetic substrates for the primary reaction. Substrates for ribonuclease S conjugate enzyme are of the formula R-X where R is a pyrimidine 3'-phosphate moiety and X is a leaving group linked to R through the 3'-phosphate group and leads to said coenzyme or prosthetic group, e.g. via riboflavin, thiamine, pyridoxal, pyridoxine or pyridoxine phosphate.

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