Episomal vector for the expression of selected dna fragments...

C - Chemistry – Metallurgy – 12 – N

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195/1.16, 195/1.

C12N 15/85 (2006.01) C07K 14/11 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/67 (2006.01) C12N 15/869 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1300052

ABSTRACT OF THE DISCLOSURE The present invention is directed to an episomal expression vector with an efficient expression potential of cloned DNA sequences. The single vector plasmid of this invention joins DNA-fragments, which allows extra chromosomal replication of the vector in a wide selec- tion of host cells and fragments, which give the transfected cells resistance for a selection drug and a mammalian expression cassette comprising one of the strongest eucaryotic enhancers known at present. The vector also carries appropriate promoter and transcrip- tion processing signals. This new combination provides this expression vector with several properties that makes it optimal to be used in transfection of cell lines suitable for production and expression of foreign cDNAs joined into this vector with high efficiency.

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