Epitope protection assay and method for detecting protein...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/02 (2006.01) A61K 39/395 (2006.01) C07K 7/08 (2006.01) C07K 16/40 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2536305

The invention relates to an epitope protection assay for use in diagnosis, prognosis and therapeutic intervention in diseases, for example, involving polypeptide aggregation, such as prion infections. The methods of the invention first block accessible polypeptide target epitope with a blocking agent. After denaturation of the polypeptide, a detecting agent is used to detect protein with target epitope that was inaccessible during contact with the blocking agent.

L'invention concerne un dosage de protection d'épitope destiné à servir dans le diagnostic, le pronostic et l'intervention thérapeutique dans le cas de maladies qui impliquent, par exemple, l'agrégation de polypeptides, telles que des infections par le prion. Les procédés selon l'invention permettent de bloquer en premier lieu l'épitope cible de polypeptide accessible au moyen d'un agent bloquant. Après dénaturation du polypeptide, on utilise un agent de détection pour détecter la protéine renfermant l'épitope cible qui était accessible lors du contact avec l'agent bloquant.

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